Publications

Preprints and Papers Under Review

Determinants that Enable Disordered Protein Assembly Into Discrete Condensed Phases

Determinants that Enable Disordered Protein Assembly Into Discrete Condensed Phases

Welles RM, Sojitra KA, Garabedian MV, Xia B, Wang W, Guan M, Regy RM, Gallagher ER, Hammer DA, Mittal J* and Good MC*. ​
Nature Chemistry (2024). DOI: 10.1038/s41557-023-01423-7
Nature Chemistry
BioRXIV preprint 

Preprints and Papers Under Review

Recent Publications

Nascent Transcriptome Reveals Orchestration of Zygotic Genome Activation In Early Embryogenesis

Nascent Transcriptome Reveals Orchestration of Zygotic Genome Activation In Early Embryogenesis

Chen H., Good, M.C.*
Current Biology 32:4314-4324 (2022). PDF. Link.

Recent Publications
Epithelial Cell Size Dysregulation In Human Lung Adenocarcinoma

Epithelial Cell Size Dysregulation In Human Lung Adenocarcinoma

Sandlin C.W., Gu S., Xu J., Deshpande C., Feldman M.D., Good M.C.*
PLoS One 17(10):e0274091 (2022). PDF. Link.

Recent Publications
Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences

Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences

Garabedian M.V., Su Z., Dabdoub J.B., Tong, M., Deiters A., Hammer D.A., Good M.C.*
Biochemistry 61(22):2470-2481 (2022). doi: 10.1021/acs.biochem.2c00250. PDF. Link

Recent Publications
Peeking Under the Hood of Early Embryogenesis: Using Tools and Synthetic Biology to Understand Native Control Systems and Sculpt Tissues

Peeking Under the Hood of Early Embryogenesis: Using Tools and Synthetic Biology to Understand Native Control Systems and Sculpt Tissues

Qian, W., Good, M.C.*
Seminars in Cell and Developmental Biology 141:43-49 (2023) . PDF. Link

Recent Publications
Co-assembly of liposomes, Dendrimersomes, and Polymersomes with amphiphilic Janus dendrimers conjugated to Mono- and Tris-Nitrilotriacetic Acid (NTA, TrisNTA) enhances protein recruitment

Co-assembly of liposomes, Dendrimersomes, and Polymersomes with amphiphilic Janus dendrimers conjugated to Mono- and Tris-Nitrilotriacetic Acid (NTA, TrisNTA) enhances protein recruitment

Xiao Q., Rivera-Martinez N., Raab C.J., Bermudez J.G., Good M.C., Klein M.L., Percec V.
Giant 9, 100089 (2022). doi: 10.1016/j.giant.2021.100089. Link

Recent Publications
Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates

Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates

Guan M., Garabedian M.V., Leutenegger M., Schuster B.S., Good, M.C., Hammer, D.A.
Biochemistry 60: 3137-3151 (2021).. Link

Recent Publications

OptoLRP6 Illuminates Wnt Signaling in Early Embryo Development

Garabedian M.V., Good M.C.*
Journal of Molecular Biology 433:167053 (2021). PDF. Link.

Recent Publications
Designer Membraneless Organelles Sequester Native Factors for Control of Cell Behavior

Designer Membraneless Organelles Sequester Native Factors for Control of Cell Behavior

Garabedian M.V., Wang W., Dabdoub J.B., Tong, M., Caldwell R.M., Benman W., Schuster B.S., Deiters A., Good, M.C.*
Nature Chemical Biology 17: 998-1007 (2021). PDF. Link.
Press: News, Views. Highlight. News. Modus Operandi

Recent Publications
Patterning Microtubule Network Organization Reshapes Cell-Like Compartments​

Patterning Microtubule Network Organization Reshapes Cell-Like Compartments​

Bermudez, J.G., Deiters A., Good, M.C.*
ACS Synthetic Biology 10:1338-1350 (2021). PDF. Link.

Recent Publications
A C. elegans Zona Pellucida domain protein functions via its ZPc domain

A C. elegans Zona Pellucida domain protein functions via its ZPc domain

Cohen J.D., Bermudez J.G., Good M.C., Sundaram M.V.
PLoS Genetics 16: e1009188 (2020). Link

Recent Publications
Integrating cellular dimensions with cell differentiation during early development

Integrating cellular dimensions with cell differentiation during early development

Chen, H., Qian, W., Good, M.C.*
Current Opinion in Cell Biology 67: 109-117 (2020). PDF. Link.

Recent Publications
Nuclear sizER in Early Development

Nuclear sizER in Early Development

Chen, H., Good, M.C.*
Developmental Cell 54: 297-298 (2020). ​PDF

Recent Publications
Direct Visualization of Vesicle Disassembly and Reassembly Using Photocleavable Dendrimers Elucidates Cargo Release Mechanisms

Direct Visualization of Vesicle Disassembly and Reassembly Using Photocleavable Dendrimers Elucidates Cargo Release Mechanisms

Li, S., Xia, B., Javed, B., Hasley, W.D., Melendez-Davila, A., Liu, M., Kerzner, M., Agarwal, S., Xiao, Q., Torre, P., Bermudez, J.G., Rahimi, K., Kostina, N.Y., Möller, M., Rodriguez-Emmenegger,C., Klein, M.L., Percec, V.* and Good, M.C.*
ACS Nano 14: 7398-7411 (2020)​. PDF. Link.

Recent Publications
Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior

Identifying Sequence Perturbations to an Intrinsically Disordered Protein that Determine Its Phase Separation Behavior

Schuster B.S., Dignon G.L., Tang W.S., Kelley F.M., Ranganath A.K., Jahnke C.N., Simpkins A.G., Regy R.M., Hammer D.A., Good M.C., Mittal J
PNAS 117: 1421-11431 (2020). PDF. Link.

Recent Publications
Imaging Nascent Transcription in Wholemount Vertebrate Embryos to Characterize Zygotic Genome Activation

Imaging Nascent Transcription in Wholemount Vertebrate Embryos to Characterize Zygotic Genome Activation

Chen, H., Good, M.C.*
Methods In Enzymology 638: 139-165​ (2020). PDF. Link.

Recent Publications
SPLIT: Stable Protein Coacervation Using a Light Induced Transition

SPLIT: Stable Protein Coacervation Using a Light Induced Transition

Reed, E.H., Schuster, B.S., Good, M.C., Hammer, D.A.
ACS Synthetic Biology 9:500-507 (2020). PDF. Link.

Recent Publications
Encapsulation of Hydrophobic Components in Dendrimersomes and Decoration of their Surface with Proteins and Nucleic Acids

Encapsulation of Hydrophobic Components in Dendrimersomes and Decoration of their Surface with Proteins and Nucleic Acids

Torre, P., Xiao, Q., Buzzacchera, I., Sherman, S.E., Rahimi, K., Kostina, N.Y., Rodriguez-Emmenegger, C., Möller, M., Wilson, C.J., Klein, M.L.*, Good, M.C.*, Percec, V.*
PNAS 116(31):15378-15385 (2019). PDF. Link.
Press: News

Recent Publications
Spatiotemporal Patterning of Zygotic Genome Activation in A Model Vertebrate Embryo

Spatiotemporal Patterning of Zygotic Genome Activation in A Model Vertebrate Embryo

Chen, H., Einstein, L.C., Little, S., Good, M.C.*
​Developmental Cell 49: 852-866 (2019). PDF. Link. Supp.
Press: News

Recent Publications

Encoding Biological Recognition In A Bicomponent Cell-Membrane Mimic

Rodriguez-Emmenegger C., Xiao Q., Kostina N.Y., Sherman S.E., Rahimi K., Partridge B.E., Li S., Sahoo D., Reveron Perez A.M., Buzzacchera I., Han H., Kerzner M., Malhotra I., Möller M., Wilson C.J., Good M.C., Goulian M., Baumgart T., Klein M.L., Percec V.
PNAS 116: 5376-5382 (2019). Link

Recent Publications
Cell Parts to Complex Processes, From the Bottom Up

Cell Parts to Complex Processes, From the Bottom Up

Good M.*, Trepat X.*
Nature 563(7730): 188-189 (2018). PDF. Link

Recent Publications
Controllable Protein Phase Separation and Modular Recruitment to Form Responsive Membraneless Organelles

Controllable Protein Phase Separation and Modular Recruitment to Form Responsive Membraneless Organelles

Schuster, B.S., Reed, E.H., Parthasarathy, R., Jahnke, C.N., Caldwell, R.M., Bermudez J.G., Ramage H.R., Good, M.C.* and Hammer, D.A.*
Nature Communications 9:2985 (2018). PDF. Link

Recent Publications
Preparation of Cellular Extracts from Xenopus Eggs and Embryos

Preparation of Cellular Extracts from Xenopus Eggs and Embryos

Good M.C.*, Heald R.*
Cold Spring Harbor Protocols (2018). PDF. Pubmed

Recent Publications
Optochemical Control of Protein Localization and Activity Within Cell-Like Compartments

Optochemical Control of Protein Localization and Activity Within Cell-Like Compartments

Caldwell R.M.#, Bermudez J.G.#, Thai D., Aonbangkhen C., Schuster B.S., Courtney T., Deiters A., Hammer D.A., Chenoweth D.M., and Good M.C.*
Biochemistry 57, 2590-2596 (2018). PDF. Pubmed

Recent Publications
Probing the Biology of Cell Boundary Conditions Through Confinement of Xenopus Cell-Free Cytoplasmic Extracts

Probing the Biology of Cell Boundary Conditions Through Confinement of Xenopus Cell-Free Cytoplasmic Extracts

Bermudez J.G., Chen H., Einstein L.C., Good M.C.*
Genesis 55 (1-2) (2017). PDF. Pubmed

Recent Publications
Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions In Synthetic Cell-like Compartments with Tunable Size

Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions In Synthetic Cell-like Compartments with Tunable Size

Good M.C.*
Methods in Molecular Biology 1413, 87-108 (2016). PDF. Pubmed

Recent Publications

Size Regulation: Big Insights from Little Cells

Chen H., and Good M.C.*
Developmental Cell 37, 392-394 (2016). PDF. Pubmed

Recent Publications

Turn Up the Volume: Uncovering Nucleus Size Control Mechanisms

Good M.C.*
Developmental Cell 33, 496-497 (2015). PDF. Pubmed

Recent Publications

Previous Publications (Prior to Penn)

A Comparative Analysis of Spindle Morphometrics across Metazoans

A Comparative Analysis of Spindle Morphometrics across Metazoans

Crowder, M.E., Strzelecka, M., Wilbur, J.D., Good, M.C., von Dassow, G., and Heald, R.
Current Biology 25, 1542-1550 (2015). PDF. Pubmed

Previous Publications (Prior to Penn)
Cytoplasmic Volume Modulates Spindle Size During Embryogenesis

Cytoplasmic Volume Modulates Spindle Size During Embryogenesis

Good, M.C., Vahey, M.D., Skandarajah, A., Fletcher, D.A., and Heald, R.
Science 342, 856-860 (2013). PDF. Pubmed.
News: Commentary

Previous Publications (Prior to Penn)
Scaffold proteins: hubs for controlling the flow of cellular information

Scaffold proteins: hubs for controlling the flow of cellular information

Good, M.C., Zalatan, J.G., and Lim, W.A.
Science 332, 680-686 (2011). PDF. Pubmed

Previous Publications (Prior to Penn)

Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB

Lombana, T.N., Echols, N., Good, M.C., Thomsen, N.D., Ng, H.L., Greenstein, A.E., Falick, A.M., King, D.S., and Alber, T.
Structure 18, 1667-1677 (2010). PDF. Pubmed

Previous Publications (Prior to Penn)

Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs

Mok, J., Kim, P.M., Lam, H.Y., Piccirillo, S., Zhou, X., Jeschke, G.R., Sheridan, D.L., Parker, S.A., Desai, V., Jwa, M., Cameroni, E., Niu, H., Good, M., Remenyi, A., Ma, J.L., Sheu, Y.J., Sassi, H.E., Sopko, R., Chan, C.S., De Virgilio, C., Hollingsworth, N.M., Lim, W.A., Stern, D.F., Stillman, B., Andrews, B.J., Gerstein, M.B., Snyder, M., and Turk, B.E.
Science Signaling 3, ra12 (2010). PDF. Pubmed

Previous Publications (Prior to Penn)
The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation

The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation

Good, M., Tang, G., Singleton, J., Remenyi, A., and Lim, W.A.
Cell 136, 1085-1097 (2009). PDF. Pubmed.
News: Commentary

Previous Publications (Prior to Penn)

The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans

King, N., Westbrook, M.J., Young, S.L., Kuo, A., Abedin, M., Chapman, J., Fairclough, S., Hellsten, U., Isogai, Y., Letunic, I., Marr, M., Pincus, D., Putnam, N., Rokas, A., Wright, K.J., Zuzow, R., Dirks, W., Good, M., … and Rokhsar, D.
Nature 451, 783-788 (2008). PDF. Pubmed

Previous Publications (Prior to Penn)

Docking interactions in protein kinase and phosphatase networks

Remenyi, A., Good, M.C., and Lim, W.A.
Current Opinion in Structural Biology 16, 676-685 (2006). PDF. Pubmed

Previous Publications (Prior to Penn)

The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway

Bhattacharyya, R.P., Remenyi, A., Good, M.C., Bashor, C.J., Falick, A.M., and Lim, W.A.
Science 311, 822-826 (2006). PDF. Pubmed.
News: Commentary

Previous Publications (Prior to Penn)
The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network

The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network

Remenyi, A., Good, M.C., Bhattacharyya, R.P., and Lim, W.A.
Molecular Cell 20, 951-962 (2005). PDF. Pubmed

Previous Publications (Prior to Penn)

An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase

Pullen, K.E., Ng, H.L., Sung, P.Y., Good, M.C., Smith, S.M., and Alber, T.
Structure 12, 1947-1954 (2004). PDF. Pubmed.
News: Commentary

Previous Publications (Prior to Penn)
Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller

Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller

Good, M.C., Greenstein, A.E., Young, T.A., Ng, H.L., and Alber, T.
Journal of Molecular Biology 339, 459-469 (2004). PDF. Pubmed

Previous Publications (Prior to Penn)